Composite
Part:BBa_K3868095:Design
Designed by: Yan Xu Group: iGEM21_NNU-China (2021-10-16)
pCas
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal PstI site found at 3473
Illegal PstI site found at 3720 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal NheI site found at 1331
Illegal PstI site found at 3473
Illegal PstI site found at 3720 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal BamHI site found at 2408 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal PstI site found at 3473
Illegal PstI site found at 3720 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1572
Illegal EcoRI site found at 2469
Illegal EcoRI site found at 3978
Illegal PstI site found at 3473
Illegal PstI site found at 3720
Illegal AgeI site found at 3900 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Gene is from S. pyogenes for the pCas plasmid of the CRISPR-Cas9 system.